modft lt program Search Results


modfit lt software macintosh  (Becton Dickinson)
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Becton Dickinson modfit lt software macintosh
Modfit Lt Software Macintosh, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell quest software  (Becton Dickinson)
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Becton Dickinson cell quest software
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modfit lt software  (Becton Dickinson)
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Becton Dickinson modfit lt software
Modfit Lt Software, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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modfit lt  (Verity Software House)
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Figure 1. Ridaforolimus attenuates mTOR signaling, resulting in cell shrinkage, cytostatic, and metabolic effects. A, to evaluate target inhibition, HT-1080 cells were treated with increasing concentrations of ridaforolimus for 2 hours before harvest and immunoblotting to assess levels of phosphorylated ribosomal protein S6 (p-S6) and 4E-BP1 (p-4E-BP1). GAPDH was included as a loading control. B, representative FSC-H graph with a plot of HT-1080 cells treated with 100 nmol/L ridaforolimus for 72 hours overlaid with a plot from untreated control cells. Mean FSC-H values for untreated and ridaforolimus-treated cells were 89 and 69, respectively. C, HT-1080 cells were incubated in the presence of 100 nmol/L ridaforolimus or vehicle control for 24 hours. Cells were harvested, stained with propidium iodide, and analyzed by flow cytometry to determine DNA content. The percentage of cells in G1-, S-, 100 or G2-M phase was calculated from FL-2 histograms using <t>ModFit</t> <t>Lt</t> <t>software.</t> *, P < 0.003. D, HT-1080 cells were treated with vehicle, 0.01, 0.1, 1.0, 10, or 100 nmol/L ridaforolimus for 48 hours. For glucose uptake, cells were exposed for 2 hours to the fluorescent glucose analogue 2-NBDG. The level of uptake of this analogue after 2-hour incubation was determined by FACS as the geometric mean of fluorescence intensity of the cell population for each sample. For GLUT1 surface expression, cells were incubated with enhanced green fluorescent protein-conjugated GLUT1 ligand for 30 minutes and the level of binding determined by FACS as above. The relative percent glucose uptake and GLUT1 expression presented was calculated as the ratio of the geometric means of drug treated versus vehicle treated cells. Inset, HT-1080 cells were treated with 100 nmol/L ridaforolimus for 72 hours and the expression levels of HIF-1a, GLUT1, GLUT4, and p-S6 determined by immunoblot.
Modfit Lt, supplied by Verity Software House, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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modfit lt software version 3.3  (Becton Dickinson)
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Becton Dickinson modfit lt software version 3.3
Figure 1. Ridaforolimus attenuates mTOR signaling, resulting in cell shrinkage, cytostatic, and metabolic effects. A, to evaluate target inhibition, HT-1080 cells were treated with increasing concentrations of ridaforolimus for 2 hours before harvest and immunoblotting to assess levels of phosphorylated ribosomal protein S6 (p-S6) and 4E-BP1 (p-4E-BP1). GAPDH was included as a loading control. B, representative FSC-H graph with a plot of HT-1080 cells treated with 100 nmol/L ridaforolimus for 72 hours overlaid with a plot from untreated control cells. Mean FSC-H values for untreated and ridaforolimus-treated cells were 89 and 69, respectively. C, HT-1080 cells were incubated in the presence of 100 nmol/L ridaforolimus or vehicle control for 24 hours. Cells were harvested, stained with propidium iodide, and analyzed by flow cytometry to determine DNA content. The percentage of cells in G1-, S-, 100 or G2-M phase was calculated from FL-2 histograms using <t>ModFit</t> <t>Lt</t> <t>software.</t> *, P < 0.003. D, HT-1080 cells were treated with vehicle, 0.01, 0.1, 1.0, 10, or 100 nmol/L ridaforolimus for 48 hours. For glucose uptake, cells were exposed for 2 hours to the fluorescent glucose analogue 2-NBDG. The level of uptake of this analogue after 2-hour incubation was determined by FACS as the geometric mean of fluorescence intensity of the cell population for each sample. For GLUT1 surface expression, cells were incubated with enhanced green fluorescent protein-conjugated GLUT1 ligand for 30 minutes and the level of binding determined by FACS as above. The relative percent glucose uptake and GLUT1 expression presented was calculated as the ratio of the geometric means of drug treated versus vehicle treated cells. Inset, HT-1080 cells were treated with 100 nmol/L ridaforolimus for 72 hours and the expression levels of HIF-1a, GLUT1, GLUT4, and p-S6 determined by immunoblot.
Modfit Lt Software Version 3.3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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modfit lt workshop program bd immunocytometry system  (Becton Dickinson)
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Becton Dickinson modfit lt workshop program bd immunocytometry system
Figure 1. Ridaforolimus attenuates mTOR signaling, resulting in cell shrinkage, cytostatic, and metabolic effects. A, to evaluate target inhibition, HT-1080 cells were treated with increasing concentrations of ridaforolimus for 2 hours before harvest and immunoblotting to assess levels of phosphorylated ribosomal protein S6 (p-S6) and 4E-BP1 (p-4E-BP1). GAPDH was included as a loading control. B, representative FSC-H graph with a plot of HT-1080 cells treated with 100 nmol/L ridaforolimus for 72 hours overlaid with a plot from untreated control cells. Mean FSC-H values for untreated and ridaforolimus-treated cells were 89 and 69, respectively. C, HT-1080 cells were incubated in the presence of 100 nmol/L ridaforolimus or vehicle control for 24 hours. Cells were harvested, stained with propidium iodide, and analyzed by flow cytometry to determine DNA content. The percentage of cells in G1-, S-, 100 or G2-M phase was calculated from FL-2 histograms using <t>ModFit</t> <t>Lt</t> <t>software.</t> *, P < 0.003. D, HT-1080 cells were treated with vehicle, 0.01, 0.1, 1.0, 10, or 100 nmol/L ridaforolimus for 48 hours. For glucose uptake, cells were exposed for 2 hours to the fluorescent glucose analogue 2-NBDG. The level of uptake of this analogue after 2-hour incubation was determined by FACS as the geometric mean of fluorescence intensity of the cell population for each sample. For GLUT1 surface expression, cells were incubated with enhanced green fluorescent protein-conjugated GLUT1 ligand for 30 minutes and the level of binding determined by FACS as above. The relative percent glucose uptake and GLUT1 expression presented was calculated as the ratio of the geometric means of drug treated versus vehicle treated cells. Inset, HT-1080 cells were treated with 100 nmol/L ridaforolimus for 72 hours and the expression levels of HIF-1a, GLUT1, GLUT4, and p-S6 determined by immunoblot.
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modfit lt tm 3.0 dna analysis software  (Becton Dickinson)
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Becton Dickinson modfit lt tm 3.0 dna analysis software
Figure 1. Ridaforolimus attenuates mTOR signaling, resulting in cell shrinkage, cytostatic, and metabolic effects. A, to evaluate target inhibition, HT-1080 cells were treated with increasing concentrations of ridaforolimus for 2 hours before harvest and immunoblotting to assess levels of phosphorylated ribosomal protein S6 (p-S6) and 4E-BP1 (p-4E-BP1). GAPDH was included as a loading control. B, representative FSC-H graph with a plot of HT-1080 cells treated with 100 nmol/L ridaforolimus for 72 hours overlaid with a plot from untreated control cells. Mean FSC-H values for untreated and ridaforolimus-treated cells were 89 and 69, respectively. C, HT-1080 cells were incubated in the presence of 100 nmol/L ridaforolimus or vehicle control for 24 hours. Cells were harvested, stained with propidium iodide, and analyzed by flow cytometry to determine DNA content. The percentage of cells in G1-, S-, 100 or G2-M phase was calculated from FL-2 histograms using <t>ModFit</t> <t>Lt</t> <t>software.</t> *, P < 0.003. D, HT-1080 cells were treated with vehicle, 0.01, 0.1, 1.0, 10, or 100 nmol/L ridaforolimus for 48 hours. For glucose uptake, cells were exposed for 2 hours to the fluorescent glucose analogue 2-NBDG. The level of uptake of this analogue after 2-hour incubation was determined by FACS as the geometric mean of fluorescence intensity of the cell population for each sample. For GLUT1 surface expression, cells were incubated with enhanced green fluorescent protein-conjugated GLUT1 ligand for 30 minutes and the level of binding determined by FACS as above. The relative percent glucose uptake and GLUT1 expression presented was calculated as the ratio of the geometric means of drug treated versus vehicle treated cells. Inset, HT-1080 cells were treated with 100 nmol/L ridaforolimus for 72 hours and the expression levels of HIF-1a, GLUT1, GLUT4, and p-S6 determined by immunoblot.
Modfit Lt Tm 3.0 Dna Analysis Software, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 1. Ridaforolimus attenuates mTOR signaling, resulting in cell shrinkage, cytostatic, and metabolic effects. A, to evaluate target inhibition, HT-1080 cells were treated with increasing concentrations of ridaforolimus for 2 hours before harvest and immunoblotting to assess levels of phosphorylated ribosomal protein S6 (p-S6) and 4E-BP1 (p-4E-BP1). GAPDH was included as a loading control. B, representative FSC-H graph with a plot of HT-1080 cells treated with 100 nmol/L ridaforolimus for 72 hours overlaid with a plot from untreated control cells. Mean FSC-H values for untreated and ridaforolimus-treated cells were 89 and 69, respectively. C, HT-1080 cells were incubated in the presence of 100 nmol/L ridaforolimus or vehicle control for 24 hours. Cells were harvested, stained with propidium iodide, and analyzed by flow cytometry to determine DNA content. The percentage of cells in G1-, S-, 100 or G2-M phase was calculated from FL-2 histograms using <t>ModFit</t> <t>Lt</t> <t>software.</t> *, P < 0.003. D, HT-1080 cells were treated with vehicle, 0.01, 0.1, 1.0, 10, or 100 nmol/L ridaforolimus for 48 hours. For glucose uptake, cells were exposed for 2 hours to the fluorescent glucose analogue 2-NBDG. The level of uptake of this analogue after 2-hour incubation was determined by FACS as the geometric mean of fluorescence intensity of the cell population for each sample. For GLUT1 surface expression, cells were incubated with enhanced green fluorescent protein-conjugated GLUT1 ligand for 30 minutes and the level of binding determined by FACS as above. The relative percent glucose uptake and GLUT1 expression presented was calculated as the ratio of the geometric means of drug treated versus vehicle treated cells. Inset, HT-1080 cells were treated with 100 nmol/L ridaforolimus for 72 hours and the expression levels of HIF-1a, GLUT1, GLUT4, and p-S6 determined by immunoblot.
Facscalibur, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson modfit lt cell cycle analysis software
Figure 1. Ridaforolimus attenuates mTOR signaling, resulting in cell shrinkage, cytostatic, and metabolic effects. A, to evaluate target inhibition, HT-1080 cells were treated with increasing concentrations of ridaforolimus for 2 hours before harvest and immunoblotting to assess levels of phosphorylated ribosomal protein S6 (p-S6) and 4E-BP1 (p-4E-BP1). GAPDH was included as a loading control. B, representative FSC-H graph with a plot of HT-1080 cells treated with 100 nmol/L ridaforolimus for 72 hours overlaid with a plot from untreated control cells. Mean FSC-H values for untreated and ridaforolimus-treated cells were 89 and 69, respectively. C, HT-1080 cells were incubated in the presence of 100 nmol/L ridaforolimus or vehicle control for 24 hours. Cells were harvested, stained with propidium iodide, and analyzed by flow cytometry to determine DNA content. The percentage of cells in G1-, S-, 100 or G2-M phase was calculated from FL-2 histograms using <t>ModFit</t> <t>Lt</t> <t>software.</t> *, P < 0.003. D, HT-1080 cells were treated with vehicle, 0.01, 0.1, 1.0, 10, or 100 nmol/L ridaforolimus for 48 hours. For glucose uptake, cells were exposed for 2 hours to the fluorescent glucose analogue 2-NBDG. The level of uptake of this analogue after 2-hour incubation was determined by FACS as the geometric mean of fluorescence intensity of the cell population for each sample. For GLUT1 surface expression, cells were incubated with enhanced green fluorescent protein-conjugated GLUT1 ligand for 30 minutes and the level of binding determined by FACS as above. The relative percent glucose uptake and GLUT1 expression presented was calculated as the ratio of the geometric means of drug treated versus vehicle treated cells. Inset, HT-1080 cells were treated with 100 nmol/L ridaforolimus for 72 hours and the expression levels of HIF-1a, GLUT1, GLUT4, and p-S6 determined by immunoblot.
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Becton Dickinson modfit lt programme
Figure 1. Ridaforolimus attenuates mTOR signaling, resulting in cell shrinkage, cytostatic, and metabolic effects. A, to evaluate target inhibition, HT-1080 cells were treated with increasing concentrations of ridaforolimus for 2 hours before harvest and immunoblotting to assess levels of phosphorylated ribosomal protein S6 (p-S6) and 4E-BP1 (p-4E-BP1). GAPDH was included as a loading control. B, representative FSC-H graph with a plot of HT-1080 cells treated with 100 nmol/L ridaforolimus for 72 hours overlaid with a plot from untreated control cells. Mean FSC-H values for untreated and ridaforolimus-treated cells were 89 and 69, respectively. C, HT-1080 cells were incubated in the presence of 100 nmol/L ridaforolimus or vehicle control for 24 hours. Cells were harvested, stained with propidium iodide, and analyzed by flow cytometry to determine DNA content. The percentage of cells in G1-, S-, 100 or G2-M phase was calculated from FL-2 histograms using <t>ModFit</t> <t>Lt</t> <t>software.</t> *, P < 0.003. D, HT-1080 cells were treated with vehicle, 0.01, 0.1, 1.0, 10, or 100 nmol/L ridaforolimus for 48 hours. For glucose uptake, cells were exposed for 2 hours to the fluorescent glucose analogue 2-NBDG. The level of uptake of this analogue after 2-hour incubation was determined by FACS as the geometric mean of fluorescence intensity of the cell population for each sample. For GLUT1 surface expression, cells were incubated with enhanced green fluorescent protein-conjugated GLUT1 ligand for 30 minutes and the level of binding determined by FACS as above. The relative percent glucose uptake and GLUT1 expression presented was calculated as the ratio of the geometric means of drug treated versus vehicle treated cells. Inset, HT-1080 cells were treated with 100 nmol/L ridaforolimus for 72 hours and the expression levels of HIF-1a, GLUT1, GLUT4, and p-S6 determined by immunoblot.
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modfit lt tm software  (Verity Inc)
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Figure 1. Ridaforolimus attenuates mTOR signaling, resulting in cell shrinkage, cytostatic, and metabolic effects. A, to evaluate target inhibition, HT-1080 cells were treated with increasing concentrations of ridaforolimus for 2 hours before harvest and immunoblotting to assess levels of phosphorylated ribosomal protein S6 (p-S6) and 4E-BP1 (p-4E-BP1). GAPDH was included as a loading control. B, representative FSC-H graph with a plot of HT-1080 cells treated with 100 nmol/L ridaforolimus for 72 hours overlaid with a plot from untreated control cells. Mean FSC-H values for untreated and ridaforolimus-treated cells were 89 and 69, respectively. C, HT-1080 cells were incubated in the presence of 100 nmol/L ridaforolimus or vehicle control for 24 hours. Cells were harvested, stained with propidium iodide, and analyzed by flow cytometry to determine DNA content. The percentage of cells in G1-, S-, 100 or G2-M phase was calculated from FL-2 histograms using <t>ModFit</t> <t>Lt</t> <t>software.</t> *, P < 0.003. D, HT-1080 cells were treated with vehicle, 0.01, 0.1, 1.0, 10, or 100 nmol/L ridaforolimus for 48 hours. For glucose uptake, cells were exposed for 2 hours to the fluorescent glucose analogue 2-NBDG. The level of uptake of this analogue after 2-hour incubation was determined by FACS as the geometric mean of fluorescence intensity of the cell population for each sample. For GLUT1 surface expression, cells were incubated with enhanced green fluorescent protein-conjugated GLUT1 ligand for 30 minutes and the level of binding determined by FACS as above. The relative percent glucose uptake and GLUT1 expression presented was calculated as the ratio of the geometric means of drug treated versus vehicle treated cells. Inset, HT-1080 cells were treated with 100 nmol/L ridaforolimus for 72 hours and the expression levels of HIF-1a, GLUT1, GLUT4, and p-S6 determined by immunoblot.
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Image Search Results


Figure 1. Ridaforolimus attenuates mTOR signaling, resulting in cell shrinkage, cytostatic, and metabolic effects. A, to evaluate target inhibition, HT-1080 cells were treated with increasing concentrations of ridaforolimus for 2 hours before harvest and immunoblotting to assess levels of phosphorylated ribosomal protein S6 (p-S6) and 4E-BP1 (p-4E-BP1). GAPDH was included as a loading control. B, representative FSC-H graph with a plot of HT-1080 cells treated with 100 nmol/L ridaforolimus for 72 hours overlaid with a plot from untreated control cells. Mean FSC-H values for untreated and ridaforolimus-treated cells were 89 and 69, respectively. C, HT-1080 cells were incubated in the presence of 100 nmol/L ridaforolimus or vehicle control for 24 hours. Cells were harvested, stained with propidium iodide, and analyzed by flow cytometry to determine DNA content. The percentage of cells in G1-, S-, 100 or G2-M phase was calculated from FL-2 histograms using ModFit Lt software. *, P < 0.003. D, HT-1080 cells were treated with vehicle, 0.01, 0.1, 1.0, 10, or 100 nmol/L ridaforolimus for 48 hours. For glucose uptake, cells were exposed for 2 hours to the fluorescent glucose analogue 2-NBDG. The level of uptake of this analogue after 2-hour incubation was determined by FACS as the geometric mean of fluorescence intensity of the cell population for each sample. For GLUT1 surface expression, cells were incubated with enhanced green fluorescent protein-conjugated GLUT1 ligand for 30 minutes and the level of binding determined by FACS as above. The relative percent glucose uptake and GLUT1 expression presented was calculated as the ratio of the geometric means of drug treated versus vehicle treated cells. Inset, HT-1080 cells were treated with 100 nmol/L ridaforolimus for 72 hours and the expression levels of HIF-1a, GLUT1, GLUT4, and p-S6 determined by immunoblot.

Journal: Molecular Cancer Therapeutics

Article Title: Ridaforolimus (AP23573; MK-8669), a Potent mTOR Inhibitor, Has Broad Antitumor Activity and Can Be Optimally Administered Using Intermittent Dosing Regimens

doi: 10.1158/1535-7163.mct-10-0792

Figure Lengend Snippet: Figure 1. Ridaforolimus attenuates mTOR signaling, resulting in cell shrinkage, cytostatic, and metabolic effects. A, to evaluate target inhibition, HT-1080 cells were treated with increasing concentrations of ridaforolimus for 2 hours before harvest and immunoblotting to assess levels of phosphorylated ribosomal protein S6 (p-S6) and 4E-BP1 (p-4E-BP1). GAPDH was included as a loading control. B, representative FSC-H graph with a plot of HT-1080 cells treated with 100 nmol/L ridaforolimus for 72 hours overlaid with a plot from untreated control cells. Mean FSC-H values for untreated and ridaforolimus-treated cells were 89 and 69, respectively. C, HT-1080 cells were incubated in the presence of 100 nmol/L ridaforolimus or vehicle control for 24 hours. Cells were harvested, stained with propidium iodide, and analyzed by flow cytometry to determine DNA content. The percentage of cells in G1-, S-, 100 or G2-M phase was calculated from FL-2 histograms using ModFit Lt software. *, P < 0.003. D, HT-1080 cells were treated with vehicle, 0.01, 0.1, 1.0, 10, or 100 nmol/L ridaforolimus for 48 hours. For glucose uptake, cells were exposed for 2 hours to the fluorescent glucose analogue 2-NBDG. The level of uptake of this analogue after 2-hour incubation was determined by FACS as the geometric mean of fluorescence intensity of the cell population for each sample. For GLUT1 surface expression, cells were incubated with enhanced green fluorescent protein-conjugated GLUT1 ligand for 30 minutes and the level of binding determined by FACS as above. The relative percent glucose uptake and GLUT1 expression presented was calculated as the ratio of the geometric means of drug treated versus vehicle treated cells. Inset, HT-1080 cells were treated with 100 nmol/L ridaforolimus for 72 hours and the expression levels of HIF-1a, GLUT1, GLUT4, and p-S6 determined by immunoblot.

Article Snippet: The percentage of cells in each phase of the cell cycle was estimated from the FL2-A channel data using ModFit LT for Mac V2.0 software (Verity Software House, Inc.).

Techniques: Inhibition, Western Blot, Control, Incubation, Staining, Flow Cytometry, Software, Fluorescence, Expressing, Binding Assay